Марк П.

Биотехнолог/Химик/Хемоинформатик/Мол.Моделирование

I am a Multidisciplinary life sciences specialist with experience in biotechnology, molecular biology, pharmaceutical
production, and computational chemistry. My work experience includes organizing the upstream production process according to GMP standards, more than 3 years of work in a molecular biology laboratory, and experience in molecular modeling. I hold a Bachelor’s degree with honors in Biotechnology and an International Master’s degree in Chemistry and Chemoinformatics.

I am looking for an interesting PhD position or a position in the pharmaceutical industry, preferably in the R&D department. 

Последнее обновление резюме 03.11.2026
Адрес Lyon, France
Электронная почта Заблокировано
Соцсеть или сайт Заблокировано
Телефон +33781339456
Согласие на обработку данных Я даю cогласие на передачу персональных данных потенциальным работодателям и иным лицам, участвующим в подборе вакансий и рассмотрении моей кандидатуры, на условиях, предусмотренных <a href="https://drive.google.com/file/d/12sY30JsdH0E6ZVn3syUwfp9IG5M3kKC5/view?usp=drive_link"> Политикой обработки персональных данных</a> и <a href="https://drive.google.com/file/d/1JtzC6jo4YwG8lUk2uH3VQQvTGmM9pGQC/view?usp=sharing">соответствующим согласием</a>

Опыт

АО Генериум
Технолог участка культивирования
Окт 2021 - Мар 2022
Я работал технологом участка культивирования на производстве вакцины Спутник V. В мои обязанности входило проведение технологического процесса культивирования в соответствии с правилами GMP (решение ЕАЭС №77), изучение и составление нормативных документов. Здесь я получил опыт работы с Sartorius Stedim Single-use Bioreactors (STR 2000, STR 1000, 2 и 3 поколений) и с другим технологическим оборудованием. Также я изучил правила производства GMP и другие нормативные документы.
Сотрудник в институте «Биоинженерия» РАН учреждения ФИЦ «Фундаментальные основы биотехнологии» РАН
Младший научный сотрудник
Июл 2018 - Окт 2021
Выделение ДНК из культур микроорганизмов, проведение ПЦР и проведение последующего анализа ампликонов с помощью гель-электрофореза, пробоподготовка образцов ДНК к секвенированию на платформе MiSeq Illumina, обработка полученных данных.

Проведение множества других стандартных, лабораторных процедур.

Образование

University of Ljubljana, University of Strasbourg
Erasmus mundus master's double diploma program in chemistry and chemoinformatics
Сен 2022 - Сен 2024
During the first year, I gained skills in numerical methods using Python and statistical mechanics. In the second year, I studied a wide range of computational chemistry subjects, including chemoinformatics, biostructure and modeling, molecular dynamics simulations (CHARMM, GROMACS), machine learning and data mining in chemistry (Weka), as well as other subjects such as structure-based computer-aided drug design, drug discovery, and web technologies (SQL, HTML/CSS, and PHP).

During my master’s thesis internship, I performed molecular dynamics simulations of multivalent ligands at constant pH in GROMACS using an HPC cluster. I submitted jobs using Open MPI and analyzed results with Bash or Python scripts, which I wrote. Also, I contributed to a project dedicated to silver bacterial resistance, which aimed to determine NBFIX parameters for silver(I) ion binding to methionine or histidine residues of Sil proteins. In this work, I used the constant-pH module of GROMACS to estimate the distribution of histidine protonation states in the target
protein sequences. Results of that work were published recently (10.3390/biophysica5010007).

Additionally, during my internship, I worked with another master’s student to explore the impact of ligand flexibility on ligand-protein interactions. For this study, I selected two Farnesoid X Receptor crystal structures, each with a different ligand (PDB IDs: 4OIV and 8HD3). We submitted 100 ns simulations for each structure and obtained some interesting results, which my colleague presented at the CS3 Summer School. That work gave me valuable experience installing molecular dynamics software, preparing simulations, and mentoring.
Московский Политехнический университет
Факультет химической технологии и биотехнологии. Специальность - Биотехнология.
Сен 2016 - Авг 2020
Получил степень бакалавра со средним балом 5.

В чем вы сильны?

My skills include: 

DNA extraction from fungal and bacterial species, PCR and condition optimization, electrophoresis,
sample preparation for Illumina MiSeq and Sanger sequencing, preparation of microbiological
media, working in a laminar flow hood, E. coli transformation, primer assembly, mass spectrometry, and mass spectra data analysis using REAXYS, FTIR spectroscopy, and NGS data analysis.
Cultivating HEK293 in 1000 L and 2000 L fermentation tanks, transducing HEK293 cells with
adenoviral vectors, operating Sartorius single-use bioreactors, maintaining the upstream process in
accordance with GMP, and managing the production team.
Using GROMACS, the HPC cluster, Bash and Python scripting, and setting up constant-pH simulations of proteins and small molecules.

Расскажите о себе что-нибудь еще: публикации, конференции, хобби

During my first projects, we published several articles, and I participated in student conferences.
Publications:
1) 3’-Amino modifications enhance the antifungal properties of N4-alkyl-5-methylcytidines for
potential biocides. New Journal of Chemistry. 2022. https://doi.org/10.1039/d1nj04312a
2) Search for Efficient Chitosan-Based Fungicides to Protect the 15th–16th Centuries Tempera
Painting in Exhibits from the State Tretyakov Gallery. Microbiology. 2020.
https://doi.org/10.1134/S0026261720060193
Patent:
New n4-modified 5-methyl-2'-deoxycytidines exhibiting antimicotic activity
RU2766333C1
During my master's thesis, I made a contribution to the work of my PhD colleague:
1) Modeling the Interaction Between Silver(I) Ion and Proteins with 12-6 Lennard-Jones Potential: A
Bottom-Up Parameterization Approach.
https://doi.org/10.3390/biophysica5010007